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Binding of chrysoidine to catalase: Spectroscopy, isothermal titration calorimetry and molecular docking studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
30464 44480 2013 8 PDF Available
Title
Binding of chrysoidine to catalase: Spectroscopy, isothermal titration calorimetry and molecular docking studies
Abstract

•Chrysoidine inhibits catalase in a non-competitive manner.•No profound conformational change of catalase occurs in the presence of chrysoidine.•ITC results indicate that catalase has two sets of binding sites for chrysoidine.•Chrysoidine is located within the bottleneck in the main channel of catalase.•We report a strategy to investigate the toxicity of chrysoidine at the molecular level.

Chrysoidine is an industrial azo dye and the presence of chrysoidine in water and food has become an environmental concern due to its negative effects on human beings. In this work, the interactions between chrysoidine and bovine liver catalase (BLC) were explored. Obvious loss in catalytic activity was observed after incubation of BLC with chrysoidine, and the inhibition effect of BLC was found to be of the non-competitive type. No profound conformational change of BLC occurs in the presence of chrysoidine as revealed by UV–vis absorption, circular dichroism and fluorescence spectroscopy studies. Isothermal titration calorimetry results indicate that catalase has two sets of binding sites for chrysoidine. Further, molecular docking simulations show that chrysoidine is located within the bottleneck in the main channel of the substrate to the active site of BLC, which explain the activity inhibition of BLC by chrysoidine.

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Keywords
Proteins; Inhibition; Spectroscopy; Isothermal titration calorimetry; Thermodynamics; Molecular docking
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Binding of chrysoidine to catalase: Spectroscopy, isothermal titration calorimetry and molecular docking studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 128, 5 November 2013, Pages 35–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us