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A pilot study of the photoprotective effect of almond phytochemicals in a 3D human skin equivalent

Paper ID Volume ID Publish Year Pages File Format Full-Text
30490 44482 2013 9 PDF Available
Title
A pilot study of the photoprotective effect of almond phytochemicals in a 3D human skin equivalent
Abstract

•Feasibility of 3D skin model in evaluation of phytochemical photoprotectants.•Alpha tocopherol was absorbed in time- and dose-dependent manners.•Almond phytochemical pretreatments alleviated UVA-induced morphological changes.•Topical almond phytochemical pretreatments reduced UVA-induced apoptosis.•Overall, almond phytochemicals provided some degree of photoprotection against UVA.

UV exposure causes oxidative stress, inflammation, erythema, and skin cancer. α-Tocopherol (AT) and polyphenols (AP) present in almonds may serve as photoprotectants. Our objectives were to assess the feasibility of using a 3D human skin equivalent (HSE) in photoprotectant research and to determine photoprotection of AT and AP against UVA radiation. AT or AP was applied to medium (25 and 5 μmol/L, respectively) or topically (1 mg/cm2 and 14 μg/cm2), followed by UVA. Photodamage assessed 96 h post UVA included HSE morphology, keratinocyte proliferation, apoptosis, and differentiation. UVA induced disorganization of basal layer, alteration of epidermal development, and fibroblast loss which were alleviated by all nutrient pretreatments. UVA significantly decreased keratinocyte proliferation compared to controls, and all pretreatments tended to negate the reduction though only the medium AT effect was statistically significant (p ⩽ 0.05). UVA led to a significant 16-fold increase in apoptosis of fibroblasts compared to the control which was alleviated by topical AP pretreatment and completely negated by topical AT (p ⩽ 0.05). In conclusion, we validated the feasibility of using HSE in evaluation of photoprotectants and found that AT and AP, applied to medium or topically, provided some degree of photoprotection against UVA.

Keywords
Antioxidant; UVA; Human skin equivalent; Tocopherol; Polyphenol
First Page Preview
A pilot study of the photoprotective effect of almond phytochemicals in a 3D human skin equivalent
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 126, 5 September 2013, Pages 17–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering