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Topical hexylaminolevulinate and aminolevulinic acid photodynamic therapy: Complete arteriole vasoconstriction occurs frequently and depends on protoporphyrin IX concentration in vessel wall

Paper ID Volume ID Publish Year Pages File Format Full-Text
30491 44482 2013 7 PDF Available
Title
Topical hexylaminolevulinate and aminolevulinic acid photodynamic therapy: Complete arteriole vasoconstriction occurs frequently and depends on protoporphyrin IX concentration in vessel wall
Abstract

•Vascular responses to topical HAL and ALA based PDT are not well known.•The vasculature was imaged using confocal microscopy in a mouse skin fold chamber.•Arterioles constricted completely in 64% in HAL and 60% in ALA groups.•Vasoconstriction strongly correlated with PpIX concentration in arteriole wall.•Vasoconstriction likely influences oxygen supply and PDT outcome.

Vascular responses to photodynamic therapy (PDT) may influence the availability of oxygen during PDT and the extent of tumor destruction after PDT. However, for topical PDT vascular effects are largely unknown. Arteriole and venule diameters were measured before and after hexylaminolevulinate (HAL) and aminolevulinic acid (ALA) PDT and related to the protoporphyrin IX (PpIX) concentration in the vessel wall. A mouse skin fold chamber model and an intravital confocal microscope allowed direct imaging of the subcutaneous vessels underlying the treated area. In both HAL and ALA groups over 60% of arterioles constricted completely, while venules generally did not respond, except for two larger veins that constricted partially. Arteriole vasoconstriction strongly correlated with PpIX fluorescence intensity in the arteriole wall. Total PpIX fluorescence intensity was significantly higher for HAL than ALA for the whole area that was imaged but not for the arteriole walls. In conclusion, complete arteriole vasoconstriction occurs frequently in both HAL and ALA based topical PDT, especially when relatively high PpIX concentrations in arteriole walls are reached. Vasoconstriction will likely influence PDT effect and should be considered in studies on topical HAL and ALA-PDT. Also, our results may redefine the vasculature as a potential secondary target for topical PDT.

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Keywords
PDT; HAL; ALA; Vascular effect; PpIX distribution; Vasoconstriction
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Topical hexylaminolevulinate and aminolevulinic acid photodynamic therapy: Complete arteriole vasoconstriction occurs frequently and depends on protoporphyrin IX concentration in vessel wall
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 126, 5 September 2013, Pages 26–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us