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Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQO-induced potentially malignant oral lesions

Paper ID Volume ID Publish Year Pages File Format Full-Text
30492 44482 2013 9 PDF Available
Title
Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQO-induced potentially malignant oral lesions
Abstract

•We treated induced oral leukoplakia with 5-ALA mediated photodynamic therapy.•We analyzed the expression of cell proliferation and cell death after PDT.•We observed that cell death index increases at 6 h and decreases after 72 h.•We concluded that the interval of PDT session must be no longer than 3 days.

Fractionation can improve photodynamic therapy (PDT) efficacy for potentially malignant oral lesion treatment. The aim of this study was to demonstrate the apoptosis/proliferation index of oral keratinocytes after two sessions of topical 5-ALA-mediated PDT in 4-Nitroquinoline-1-oxide-induced potentially malignant oral lesion, and to suggest the ideal interval between PDT sessions. Immuno-histochemical tests for proliferating cell nuclear antigen and caspase-3, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed at 6 h, 24 h, 48 h, and 72 h time intervals after PDT. The number of positive cells showing caspase-3 expression was significantly higher, mainly at 6 h after PDT. In the first cycle of PDT, the highest frequency of positive cells for TUNEL was found at 24 h. At 72 h after PDT, proliferating cell nuclear antigen positive cells increased significantly, indicating that there was an epithelial response in direction towards DNA repair and cell proliferation at this time. Because cell proliferation increases and cell death index decreases at 72 h after PDT, it is recommended that the interval between the PDT sessions must not be longer than 2 days up to total lesion remission.

Keywords
Potentially malignant oral lesion; 5-ALA-mediated photodynamic therapy; Caspase-3 expression; Proliferating cell nuclear antigen expression; Immunohistochemistry
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Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQO-induced potentially malignant oral lesions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 126, 5 September 2013, Pages 33–41
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us