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Binding properties of butylated hydroxytoluene with calf thymus DNA in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
30502 44482 2013 7 PDF Available
Title
Binding properties of butylated hydroxytoluene with calf thymus DNA in vitro
Abstract

•The binding mode between BHT and ctDNA is an intercalation.•BHT most likely binds to adenine and thymine base pairs of ctDNA.•The binding of BHT to ctDNA does not alter the native B-conformation of ctDNA.•The binding is driven mainly by hydrogen bonds and van der Waals force.

The binding properties of butylated hydroxytoluene (BHT) with calf thymus DNA (ctDNA) in simulated physiological buffer (pH 7.4) were investigated using ethidium bromide (EB) dye as a fluorescence probe by various spectroscopic techniques including UV–vis absorption, fluorescence, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectroscopy along with ctDNA melting studies and viscosity measurements. It was found that the binding of BHT to ctDNA could decrease the absorption intensity of ctDNA, significantly increase melting temperature and relative viscosity of ctDNA, and induce the changes in CD spectra. Moreover, the competitive binding studies showed that BHT was able to displace EB from the bound ctDNA–EB complex. All the experimental results indicated that the binding mode between BHT and ctDNA was an intercalation. The association constants between BHT and ctDNA were evaluated to be (4.78 ± 0.04) × 103, (2.86 ± 0.02) × 103 and (1.80 ± 0.04) × 103 L mol−1 at 298, 304, 310 K, respectively. Further, the FT-IR analysis revealed that BHT was more prone to interact with adenine and thymine base pairs, and no significant conformational transition of ctDNA occurred. Thermodynamic analysis of the binding data showed that the binding process was primarily driven by hydrogen bonds and van der Waals forces, as the values of the enthalpy change and the entropy change were calculated to be −62.47 ± 0.07 kJ mol−1 and −139.22 ± 0.22 J mol−1 K−1, respectively.

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Keywords
Butylated hydroxytoluene; Food antioxidant; Calf thymus DNA; Binding mode; Circular dichroism spectroscopy; Fourier transform infrared spectroscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 126, 5 September 2013, Pages 112–118
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us