The effect of dietary soyabean isoflavones on photodynamic therapy in K562 leukemia cells
The soyabean isoflavones genistein (GEN) and daidzein (DA) are popular presented in diet. Isoflavones have a variety of biological activities including antioxidant and anticancer properties. On account of its antioxidant activity, isoflavones might protect cancer cells from free radical damage in photodynamic (PDT) during which reactive oxygen species (ROS) production was stimulated leading to irreversible tumor cell injury. In this study, the influence of GEN and DA on K562 cells in 5-aminolevulinic acid (ALA)-based PDT was demonstrated. The results showed that GEN inhibited cell proliferation and enhance cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT on K562 cells. However, DA did not enhance cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT. In conclusion, the results suggested that soy consumption during PDT did not decrease the effectiveness of cancer therapy on malignant cells.
► We deal with the effect of natural antioxidants on photodynamic therapy. ► We examine the influence of genistein and daidzein on K562 cells in PDT. ► Genistein enhances the PDT induced cytotoxicity in K562 cells. ► Daidzein did not affect the PDT efficacy in K562 cells. ► Soy consumption during PDT did not decrease the effectiveness of therapy.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 110, 2 May 2012, Pages 28–33