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Spatial arrangement of selected fluorescence labels in lipid bilayer

Paper ID Volume ID Publish Year Pages File Format Full-Text
30528 44485 2013 6 PDF Available
Title
Spatial arrangement of selected fluorescence labels in lipid bilayer
Abstract

•Energy transfer between suitable two fluorescent particles is possible.•It can be measured and described according with adequate model.•The distance between particles and its orientation factor are parameters of the model.•Both parameters according with the used model were determined.•Space orientation and location of particles in lipid bilayer were settled too.

The method for the determination the orientation factor κ2, spatial arrangement and depth position of fluorescence labels located in hydrophilic layers of vesicles bilayer from resonance energy transfer (RET) data is presented. The method is based on the broadened Wolber and Hudson RET model in two dimensions (Biophys J. 1979). The vesicles were labeled with N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (NBD-PE) as the donor and N-(Lissamine rhodamine B sulfonyl) 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (NRh-PE) as the acceptor. It was found that in basic environment sodium dithionite quenches fluorescence of both labels located in outer leaflet of bilayer. Therefore, RET data prior to and following dithionite treatment were compared and the donor–acceptor cis and trans distances of the closest approach as well as cis and trans Förster radii R0, and orientation factors κ2 for cis RET equal to 0.61 ± 0.06 and for trans RET equal to 0.17 ± 0.01 were assigned. Knowing the κ2 data, the spatial arrangement of NBD and NRh labels as dipoles in dipalmitoylphosphatidylcholine bilayer were described.

Keywords
NBD-PE; NRh-PE; Orientation factor; Sodium dithionite; Fluorescence quenching
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Spatial arrangement of selected fluorescence labels in lipid bilayer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 125, 5 August 2013, Pages 26–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us