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Electrochemical and spectroscopic investigations of carboxylic acid ligand and its triorganotin complexes for their binding with ds.DNA: In vitro biological studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
30534 44485 2013 13 PDF Available
Title
Electrochemical and spectroscopic investigations of carboxylic acid ligand and its triorganotin complexes for their binding with ds.DNA: In vitro biological studies
Abstract

•Synthesis characterization of carboxylic acid ligand and its triorganotin complexes.•All compounds interact with DNA via intercalation.•Among all comparatively good DNA binding was investigated for APA-3 complex.•APA-3 complex showed better antitumor potential.

A carboxylic acid ligand, (Z)-4-(4-acetylphenylamino)-4-oxobut-2-enoic acid (APA-1), and its triphenyl-(APA-2) and tributyl-tin(IV) (APA-3) compounds have been synthesized and investigated for their binding with ds.DNA using UV–visible spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and viscosity measurements under physiological conditions of pH and temperature. The experimental results from all techniques i.e. binding constant (Kb), binding site size (n) and free energy change (ΔG) were in good agreement and inferred spontaneous compound–DNA complexes formation via intercalation. Among all the compounds APA-3 showed comparatively greater binding at pH 4.7 as evident from its greater Kb values {APA-3: Kb: 5.63 × 104 M−1 (UV); 7.94 × 104 M−1 (fluorescence); 9.91 × 104 M−1 (CV)}. Electrochemical processes of compounds before and after the addition of DNA were found diffusion controlled. Among all compounds, APA-3 exhibited best antitumor activity.

Graphical abstractCarboxylic acid ligand and its triorganotin complexes; investigated for their binding with DNA and bioactivities. Among all tri-n-butyltin complex showed comparatively good DNA binding via intercalation and better antitumor potential.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Triorganotin(IV) carboxylates; UV–visible/fluorescence spectroscopy; Cyclic voltammerty; Intercalation; Binding constant/site size; Bioactivities
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Electrochemical and spectroscopic investigations of carboxylic acid ligand and its triorganotin complexes for their binding with ds.DNA: In vitro biological studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 125, 5 August 2013, Pages 70–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us