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Purification and characterization of a novel thermostable luciferase from Benthosema pterotum

Paper ID Volume ID Publish Year Pages File Format Full-Text
30541 44485 2013 6 PDF Available
Title
Purification and characterization of a novel thermostable luciferase from Benthosema pterotum
Abstract

•A novel thermostable luciferase from Benthosema pterotum (BP) was purified.•The molecular mass of novel enzyme is about 27 kDa and its Km value is 0.4 ± 0.01 μM.•BP luciferase showed maximum intensity of emitted light at 40 °C, pH 9 and 30 mM magnesium.•BP luciferase is a thermostable enzyme and it shows a high activity at extreme pH values.•BP luciferases generated blue light with a maximum emission wavelength at 475 nm.

A novel luciferase from Benthosema pterotum, collected from Port of Jask, close to Persian Gulf, was purified for the first time, using Q-Sepharose anion exchange chromatography. The molecular mass of the novel enzyme, measured by SDS–PAGE technique, was about 27 kDa and its Km value is 0.4 μM; both values are similar to those of other coelenterazine luciferases. B. pterotum (BP) luciferase showed maximum intensity of emitted light at 40 °C, in 20 mM Tris buffer, pH 9 and 20 mM magnesium concentration. Experimental measurements indicated that BP luciferase is a relatively thermostable enzyme; furthermore it shows a high residual activity at extreme pH values. Its biological activity is strongly inhibited by 1 mM Cu2+, Zn2+ and Ni2+, while calcium and mainly magnesium ions strongly increase BP luciferase activity. The B. pterotum luciferase generated blue light with a maximum emission wavelength at 475 nm and showed some similarity with other luciferases, while other parameters appeared quite different, in this way, confirming that a novel protein has been purified.

Keywords
Benthosema pterotum luciferase; Enzyme purification; Bioluminescence; Persian Gulf
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Purification and characterization of a novel thermostable luciferase from Benthosema pterotum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 125, 5 August 2013, Pages 131–136
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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