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Hypericin encapsulated in solid lipid nanoparticles: Phototoxicity and photodynamic efficiency

Paper ID Volume ID Publish Year Pages File Format Full-Text
30543 44485 2013 9 PDF Available
Title
Hypericin encapsulated in solid lipid nanoparticles: Phototoxicity and photodynamic efficiency
Abstract

•Hypericin was encapsulated in SLN with no need of solvent use.•Encapsulated Hypericin improved the dispersion in aqueous system.•Hy-loaded SLN has improved the photostability.•Hy-SLN increased the production of 1O2, most likely due to lower aggregation.•Hy-SLN increased the intracellular accumulation and phototoxicity.

The hydrophobicity of some photosensitizers can induce aggregation in biological systems, which consequently reduces photodynamic activity. The conjugation of photosensitizers with nanocarrier systems can potentially be used to overcome this problem. The objective of this study was to prepare and characterise hypericin-loaded solid lipid nanoparticles (Hy-SLN) for use in photodynamic therapy (PDT). SLN were prepared using the ultrasonication technique, and their physicochemical properties were characterised. The mean particle size was found to be 153 nm, with a low polydispersity index of 0.28. One of the major advantages of the SLN formulation is its high entrapment efficiency (EE%). Hy-SLN showed greater than 80% EE and a drug loading capacity of 5.22% (w/w). To determine the photodynamic efficiency of Hy before and after encapsulation in SLN, the rate constants for the photodecomposition of two 1O2 trapping reagents, DPBF and AU, were determined. These rate constants exhibited an increase of 60% and 50% for each method, respectively, which is most likely due to an increase in the lifetime of the triplet state caused by the increase in solubility. Hy-SLN presented a 30% increase in cell uptake and a correlated improvement of 26% in cytotoxicity. Thus, all these advantages suggest that Hy-loaded SLN has potential for use in PDT.

Keywords
Drug delivery system; Biocompatible nanoparticles; Encapsulation; Photodynamic therapy; Cancer cells; Hypericin
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Hypericin encapsulated in solid lipid nanoparticles: Phototoxicity and photodynamic efficiency
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 125, 5 August 2013, Pages 146–154
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us