fulltext.study @t Gmail

A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system

Paper ID Volume ID Publish Year Pages File Format Full-Text
30548 44485 2013 6 PDF Available
Title
A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system
Abstract

•First report of the expression of a eukaryotic rhodopsin in a cell-free system.•Identification of the second rhodopsin-like gene in Cyanophora paradoxa.•Sequencing of the second rhodopsin-like gene in C. paradoxa.•Absorption spectrum of the second rhodopsin in C. paradoxa.

Here we report the identification and expression of a second rhodopsin-like protein in the alga Cyanophora paradoxa (Glaucophyta), named Cyanophopsin_2. This new protein was identified due to a serendipity event, since the RACE reaction performed to complete the sequence of Cyanophopsin_1, (the first rhodopsin-like protein of C. paradoxa identified in 2009 by our group), amplified a 619 bp sequence corresponding to a portion of a new gene of the same protein family. The full sequence consists of 1175 bp consisting of 849 bp coding DNA sequence and 4 introns of 326 bp. The protein is characterized by an N-terminal region of 47 amino acids, followed by a region with 7 α-helices of 213 amino acids and a C-terminal region of 22 amino acids. This protein showed high identity with Cyanophopsin_1 and other rhodopsin-like proteins of Archea, Bacteria, Fungi and Algae. Cyanophosin_2 (CpR2) was expressed in a cell-free expression system, and characterized by means of absorption spectroscopy.

Keywords
Rhodopsin-like protein; Cyanophora paradoxa; CpR2; Cell-free expression; Absorption spectrum
First Page Preview
A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 125, 5 August 2013, Pages 188–193
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us