fulltext.study @t Gmail

Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
30554 44486 2008 6 PDF Available
Title
Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis
Abstract

Photoprocesses of 1,4-naphthoquinone (NQ) and its photoreactions with lysozyme in acetonitrile/water (3:1, v/v) solution were studied using 355 nm laser flash photolysis technique combined with electrophoresis and turbidimetric assay. The transient spectra of NQ were observed and the transient species were assigned. The electron transfer process from N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) to NQ triplet state (3NQ∗) was investigated and the rate constant was determined to be kt1 = 2.0 × 1010 M−1 s−1. It has been found that 3NQ∗ can abstract hydrogen atom from lysozyme with a rate constant of kt2 = 2.4 × 1010 M−1 s−1. Furthermore, the results of steady-state analysis suggested that lysozyme can be damaged by NQ irradiated with UVA light influenced by the concentration of NQ and the gas saturated in the solution. The mechanisms of photosensitized damage of lysozyme were discussed.

Keywords
1,4-Naphthoquinone; Lysozyme; Laser flash photolysis; Electron transfer; Hydrogen atom abstraction; Photosensitizer
First Page Preview
Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 92, Issue 2, 21 August 2008, Pages 77–82
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us