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Asymmetric distribution of biotin labeling on the purple membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
30561 44486 2008 5 PDF Available
Title
Asymmetric distribution of biotin labeling on the purple membrane
Abstract

This work examined the biotin modification of bacteriorhodopsin (BR) in the purple membrane (PM). The results of flash kinetic absorption measurements showed that photocycle was maintained in biotinylated BR. Biotinylated BR also maintained its photoelectric activity, as indicated by the photoelectric response of the bilayer lipid membrane (BLM). Atomic force microscopy (AFM) of stretavidiin-bound biotin revealed that biotin molecules covered both surfaces of the, but the amount of biotinylated BR on the extracellular (EC) surface was markedly higher than on the cytoplasmic (CP) surface. Further studies showed that, after reaction with fluorescamine (FL), biotin labeling occurred only on the CP surface. These results are informative for future work on bioconjugation of BR as well as work on oriented assembly and the design of BR-based photoelectric devices.

Keywords
Bacteriorhodopsin; Purple membrane; Biotinylation; Atomic force microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 92, Issue 2, 21 August 2008, Pages 123–127
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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