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Protective effect of (2′S)-columbianetin from Corydalis heterocarpa on UVB-induced keratinocyte damage

Paper ID Volume ID Publish Year Pages File Format Full-Text
30576 44488 2012 8 PDF Available
Title
Protective effect of (2′S)-columbianetin from Corydalis heterocarpa on UVB-induced keratinocyte damage
Abstract

A salt tolerant plant, Corydalis heterocarpa has been used as a folk medicine to treat travail and spasm. Recent studies have also reported antioxidant and antiinflammatory activities of compounds isolated from C. heterocarpa.In this study, the protective effect of (2′S)-columbianetin isolated from C. heterocarpa on UVB-induced human keratinocyte (HaCaT) damage was investigated. First, the appropriate energy level of UVB irradiation was determined using MTT and LDH assays. And then the protective effect of (2′S)-columbianetin on UVB induced HaCaT damage was evaluated by measuring; the changes in cell viability, LDH release level, ROS generation, cell cycle arrest and MMP expression levels. Finally, the effect of compound on MAPK and AP-1 signaling pathways were studied to understand the underlying signaling mechanisms.Result demonstrated that the presence of (2′S)-columbianetin suppressed the reactive oxygen species (ROS) generation, cell cycle arrest at sub-G1 phase and down regulation of MMP expression in UVB treated HaCaT cells.Furthermore, stress activated signaling pathways (ASK1-MAPK) and AP-1 signaling pathway were regulated by (2′S)-columbianetin treatment. These results suggest that (2′S)-columbianetin could be effectively used to protect human keratinocytes from UVB induced damage.

► UVB cause cellular damage to Human keratinocyte (HaCaT) cells. ► (2′S)-columbianetin attenuates intracellular ROS, sub-G1 arrest and MMP production due to UVB exposure in HaCaT cells. ► (2′S)-columbianetin suppressed cellular damage through the ASK1-MAPK and AP-1 signaling pathway. ► (2′S)-columbianetin is an affective natural compound for preventing cellular damage induced by UVB exposure.

Keywords
Corydalis heterocarpa; UVB; (2′S)-columbianetin; Photodamage; HaCaT cells
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Protective effect of (2′S)-columbianetin from Corydalis heterocarpa on UVB-induced keratinocyte damage
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 109, 2 April 2012, Pages 20–27
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us