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The effects of combined treatment with ionizing radiation and indocyanine green-mediated photodynamic therapy on breast cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
30579 44488 2012 8 PDF Available
Title
The effects of combined treatment with ionizing radiation and indocyanine green-mediated photodynamic therapy on breast cancer cells
Abstract

This study was undertaken to evaluate the effects of indocyanine green as a sensitizer in both photodynamic and radiation therapy on MCF-7 human breast cancer cells line. The cells were incubated with indocyanine green at different concentrations for 24 h and were then exposed in the independent treatment groups to a non-coherent light at different fluence rates and X-ray ionizing radiation at different dose rates. In addition, combination effects of this chemo, photo, and radiotherapy were evaluated. The percentage of the cell survival was investigated using the MTT assay. The results showed that indocyanine green had no significant cytotoxic effects up to 100 μM but as a photosensitizer had a strong cytotoxic effect on cancer cells. Despite, indocyanine green could not act as a radiosensitizer. Furthermore, it is surprising to find that 50 μM of indocyanine green in combination with light at 60 J/cm2 and 4 Gy of X-ray radiation astonishingly killed cancer cells and reduced the percentage of viable cancer cells to be 3.42%. According to the findings, we observed the same efficacy of treatment by adding a low dose of radiation and reducing light fluence rate. In fact, it appears from our data that the adverse effects of photodynamic therapy can be partially abated without reducing the efficacy of treatment. Obviously, this new therapeutic avenue in breast cancer therapy could be worth further investigation and elucidation and should be tested in vivo models for being applied in human therapy.

► The search to obtain the new and novel therapeutic avenue in cancer therapy with low side effects has been developed. ► PDT is a good alternative for cancer therapies. ► ICG (a non-toxic agent) potentially acts as a photosensitizer rather than as a radiosensitizer in MCF-7 cancer cells. ► ICG mediated PDT induces cell death acted through cytotoxic singlet oxygen. ► A combined treatment with ionizing radiation and ICG-PDT showed a potent synergistic effect.

Keywords
Ionizing radiation; Photodynamic therapy; Indocyanine green; Breast cancer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 109, 2 April 2012, Pages 42–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us