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Photophysics of DNA staining dye Propidium Iodide encapsulated in bio-mimetic micelle and genomic fish sperm DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30581 44488 2012 10 PDF Available
Title
Photophysics of DNA staining dye Propidium Iodide encapsulated in bio-mimetic micelle and genomic fish sperm DNA
Abstract

Photophysical processes in Propidium Iodide (PI), the well known DNA staining dye, have been exploited in homogeneous as well as heterogeneous medium by steady state and time resolved spectroscopy. The DNA staining dye PI exhibits intermolecular proton transfer reaction in aqueous and hydrogen-bonding acceptor solvents due to the formation of quinonoid structure which is acidic in nature. Time resolved emission spectroscopy also predicts the hydrogen bond donor ability of PI. The target dye interacts only with anionic surfactant sodium dodecylsulphate but not with cationic surfactant cetyltrimethylammonium bromide and neutral surfactant Triton X-100 and this interaction is found to be electrostatic in nature. We have further scrutinized the mode of binding of PI with fish sperm DNA. The effect of addition of urea, fluorescence quenching phenomenon, CD measurements reveal that the probe binds to DNA through intercalative style.

► Photophysics of DNA staining dye Propidium Iodide in different medium. ► Binding interaction of Propidium Iodide with micelles and a genomic fish sperm DNA. ► Study of electrostatic and intercalative type binding.

Keywords
Propidium Iodide; Fluorescence quenching; Time resolved fluorescence; Circular dichroism; Fish sperm DNA; Intercalation
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Photophysics of DNA staining dye Propidium Iodide encapsulated in bio-mimetic micelle and genomic fish sperm DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 109, 2 April 2012, Pages 58–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us