fulltext.study @t Gmail

Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer

Paper ID Volume ID Publish Year Pages File Format Full-Text
30589 44489 2013 4 PDF Available
Title
Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer
Abstract

•The structure of the D75N mutant of sensory rhodopsin II in complex with its cognate transducer is presented.•The topology of the complex is “U”-shape.•The changes in the hydrogen bonds network are observed.•The implications on the prospective mechanism of functioning are discussed.

The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.

Keywords
Membrane protein; Signal transduction; Retinal protein; X-ray crystallography; Protein crystallization
First Page Preview
Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 123, 5 June 2013, Pages 55–58
Authors
, , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us