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Comparison of the efficiency and the specificity of DNA-bound and free cationic porphyrin in photodynamic virus inactivation

Paper ID Volume ID Publish Year Pages File Format Full-Text
30595 44491 2008 8 PDF Available
Title
Comparison of the efficiency and the specificity of DNA-bound and free cationic porphyrin in photodynamic virus inactivation
Abstract

The risk of transmitting infections by blood transfusion has been substantially reduced. However, alternative methods for inactivation of pathogens in blood and its components are needed. Application of photoactivated cationic porphyrins can offer an approach to remove non-enveloped viruses from aqueous media. Here we tested the virus inactivation capability of meso-Tetrakis(4-N-methylpyridyl)porphyrin (TMPyP) and meso-Tri-(4-N-methylpyridyl)monophenylporphyrin (TMPyMPP) in the dark and upon irradiation. T7 bacteriophage, as a surrogate on non-enveloped viruses was selected as a test system. TMPyP and TMPyMPP reduce the viability of T7 phage already in the dark, which can be explained by their selective binding to nucleic acid. Both compounds proved to be efficient photosensitizers of virus inactivation. The binding of porphyrin to phage DNA was not a prerequisite of phage photosensitization, moreover, photoinactivation was more efficiently induced by free than by DNA bound porphyrin. As optical melting studies and agarose gel electrophoresis of T7 nucleoprotein revealed, photoreactions of TMPyP and TMPyMPP affect the structural integrity of DNA and also of viral proteins, despite their selective DNA binding.

Keywords
Cationic porphyrin; Photodynamic treatment; Virus inactivation; DNA-binding
First Page Preview
Comparison of the efficiency and the specificity of DNA-bound and free cationic porphyrin in photodynamic virus inactivation
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 90, Issue 2, 27 February 2008, Pages 105–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering