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Binding of fullerol to human serum albumin: Spectroscopic and electrochemical approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
30604 44492 2012 10 PDF Available
Title
Binding of fullerol to human serum albumin: Spectroscopic and electrochemical approach
Abstract

The potential impact of human exposure to carbonaceous nanomaterials in the environment becomes a concerning issue. Here we report on the interaction of fullerol with human serum albumin (HSA) using spectroscopic and electrochemical methods. The water-soluble fullerene derivative (fullerol) was synthesized and characterized by IR, 1H NMR, TG-DSC, XRD, HR-TEM, etc. The spectroscopic methods show that the fluorescence quenching of HSA by fullerol is the result of the formation of an HSA-fullerol complex. Binding parameters such as ΔG, ΔH and ΔS were calculated, and the quenching constant Ka at different temperatures was determined using the modified Stern–Volmer equation. The electrochemical experiments further confirmed the conclusions. In addition, the influences of coexisting heavy metal ions have also been studied in the present system. The circular dichroism spectra (CD), 3D fluorescence spectra and FT-IR spectra results suggest that the secondary structure of HSA was changed by fullerol. Based on the site marker competitive experiments, we can predict the possible binding position of fullerol on the HSA was located at the site of sub domain II A. Furthermore, the distance r between donor (HSA) and acceptor (fullerol) was obtained according to the famous fluorescence resonance energy transfer (FRET) mechanism.

► One water-soluble fullerene derivative fullerol was synthesized and characterized. ► Various detection methods suggest the mechanism might be a static quenching. ► The multiple hydrogen bonds and van der Waals forces play a major role in association. ► This study should help the understanding of how nanomaterials interact with proteins.

Keywords
Binding; Polyhydroxylated fullerene; Human serum albumin; Fluorescence spectroscopy; Electrochemical approach
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Binding of fullerol to human serum albumin: Spectroscopic and electrochemical approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 108, 1 March 2012, Pages 34–43
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us