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Binding of an indole alkaloid, vinblastine to double stranded DNA: A spectroscopic insight in to nature and strength of interaction

Paper ID Volume ID Publish Year Pages File Format Full-Text
30606 44492 2012 5 PDF Available
Title
Binding of an indole alkaloid, vinblastine to double stranded DNA: A spectroscopic insight in to nature and strength of interaction
Abstract

Vinblastine is a chemotherapeutic drug, used for the treatment of various cancers. It functions by interfering with DNA in fast growing cells and preventing them from reproducing. The present work is focused on the interaction of vinblastine with double stranded DNA in aqueous solution. Fourier transform infrared and UV–Visible absorption spectroscopy were used to analyze the interaction of vinblastine with calf-thymus DNA. FTIR analysis showed binding of vinblastine through A–T and G–C base pairs of DNA along with its phosphate backbone. UV–Vis spectroscopy results suggested the intercalation of drug in between the base pairs of DNA double helix. The binding constant estimated for vinblastine–DNA association was found to be K = 1.7 × 103 M−1. Molecular docking was performed and the results showed adenine base binding of vinblastine with DNA. Furthermore spectroscopic results revealed that formation of vinblastine–DNA complex resulted in no major change in the B-conformation of DNA.

Graphical abstractFTIR and UV–Vis spectroscopic studies on the binding on vinblastine with DNA showed intercalation of vinblastine in to DNA double helix along with interaction through the phosphate backbone.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Binding of vinblastine with DNA was studied using FTIR and UV absorption spectroscopy. ► Results showed intercalation of vinblastine through DNA base pairs. ► Binding with phosphate backbone is indicated with no change in B-DNA conformation. ► Binding constant for vinblastine–DNA association was found to be K = 1.7 × 103 M−1.

Keywords
DNA; Vinblastine; FTIR spectroscopy
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Binding of an indole alkaloid, vinblastine to double stranded DNA: A spectroscopic insight in to nature and strength of interaction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 108, 1 March 2012, Pages 48–52
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us