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Spectroscopic studies on the interaction between carbaryl and calf thymus DNA with the use of ethidium bromide as a fluorescence probe

Paper ID Volume ID Publish Year Pages File Format Full-Text
30607 44492 2012 9 PDF Available
Title
Spectroscopic studies on the interaction between carbaryl and calf thymus DNA with the use of ethidium bromide as a fluorescence probe
Abstract

The interaction between carbaryl and calf thymus DNA (ctDNA) was investigated under simulated physiological conditions (Tris–HCl buffer of pH 7.4) using ethidium bromide (EB) dye as a probe by UV–vis absorption, fluorescence and circular dichroism (CD) spectroscopy, as well as DNA melting studies and viscosity measurements. It can be concluded that carbaryl molecules could intercalate into the base pairs of DNA as evidenced by hyperchromic effect of absorption spectra, decreases in iodide fluorescence quenching effect, induced CD spectral changes, and significant increases in melting temperature and relative viscosity of DNA. The binding constants and thermodynamic parameters of carbaryl with DNA were obtained by the fluorescence quenching method. Furthermore, a chemometrics approach, parallel factor analysis (PARAFAC), was applied to resolve the measured three-way synchronous fluorescence spectral data matrix of the competitive interaction between carbaryl and EB with DNA, and the results provided simultaneously the concentration profiles and corresponding pure spectra for three reaction components (carbaryl, EB and DNA–EB complex) of the kinetic system at equilibrium. This PARAFAC analysis demonstrated the intercalation of carbaryl to the DNA helix by substituting for EB in the DNA–EB complex.

► The interaction between carbaryl and calf thymus DNA is investigated. ► The binding mode of carbaryl to DNA is an intercalation binding. ► The parallel factor analysis confirms the intercalation of carbaryl to the DNA helix. ► The binding is driven mainly by hydrogen bond and van der Waals forces.

Keywords
Carbaryl; Calf thymus DNA; Spectroscopy; Parallel factor analysis; Ethidium bromide
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Spectroscopic studies on the interaction between carbaryl and calf thymus DNA with the use of ethidium bromide as a fluorescence probe
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 108, 1 March 2012, Pages 53–61
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us