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Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
30609 44493 2006 8 PDF Available
Title
Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes
Abstract

UVA radiation provokes the generation of reactive oxygen species (ROS), which induce oxidative stress in the exposed cells leading to extensive cellular damage and cell death either by apoptosis or necrosis. One approach to protecting human skin against the harmful effects of UV radiation is by using herbal compounds as photoprotectants. This study evaluated the protective effects of Prunella vulgaris L. (Labiatae) and its main phenolic acid component, rosmarinic acid (RA), against UVA-induced changes in a human keratinocyte cell line (HaCaT). Human keratinocytes exposed to UVA (10–30 J/cm2) were treated with an extract of P. vulgaris (1–75 mg/l) or RA (0.9–18 mg/l) for 4 h. P. vulgaris and RA exhibited ability to reduce the UVA-caused decrease in a cell viability monitored by neutral red retention and by LDH release into medium. The P. vulgaris extract and RA significantly suppressed UVA-induced ROS production, which manifests as a decrease in intracellular lipid peroxidation, elevation of ATP and reduced glutathione. Post-treatment with P. vulgaris extract and RA also significantly reduced DNA damage. In addition, UVA-induced activation of caspase-3 was inhibited by treatment with P. vulgaris and RA.The P. vulgaris extract and RA demonstrated a concentration-dependent photoprotection (maximum at 25–50 mg/l and 9 mg/l, respectively). These results suggest that P. vulgaris and RA, used in skin care cosmetics, may offer protection against UVA-induced oxidative stress and may be beneficial as a supplement in photoprotective dermatological preparations.

Keywords
UVA radiation; Photoprotection; Prunella vulgaris; Rosmarinic acid; Keratinocytes
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Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 84, Issue 3, 1 September 2006, Pages 167–174
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us