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Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
30610 44493 2006 6 PDF Available
Title
Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli
Abstract

Allophycocyanin (APC) is the main core component of phycobilisome found in blue–green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 × His-tag and their α or β subunits can scavenge hydroxyl radicals successfully, and the separated α or β subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin.

Keywords
Antioxidant; Recombinant allophycocyanin; Escherichia coli; Anti-tumor; Hydroxyl radicals
First Page Preview
Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 84, Issue 3, 1 September 2006, Pages 175–180
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering