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A polypeptide from Chlamys farreri abolishes UV-induced apoptosis in murine thymocytes in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
30612 44493 2006 8 PDF Available
Title
A polypeptide from Chlamys farreri abolishes UV-induced apoptosis in murine thymocytes in vitro
Abstract

Previously we reported that a polypeptide from Chlamys farreri (PCF) was a potent photoprotective agent against ultraviolet (UV) irradiation in vitro. To understand the mechanism by which PCF protects cells from irradiation, we studied anti-apoptotic effects of PCF against UV irradiation on the murine thymocytes in vitro. MTT and flow cytometric analysis assays showed that 2 h pretreatment with PCF completely abolished UV induced cell death. TEM examination showed that PCF fully protected the ultrastructure of thymocytes exposed to UV irradiation. Lipid peroxidation and intracellular reactive oxygen species assays indicated that PCF efficiently blocked production of reactive oxygen intermediates induced by UV irradiation. Further, PCF protected UV-irradiated thymocytes from losing mitochondrial transmembrane potential and DNA fragmentation. Based on these observations we propose that PCF is a potent anti-apoptotic factor, which protects cells from irradiation at multiple steps.

Keywords
Polypeptide from Chlamys farreri; Ultraviolet; Apoptosis; Murine thymocytes
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A polypeptide from Chlamys farreri abolishes UV-induced apoptosis in murine thymocytes in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 84, Issue 3, 1 September 2006, Pages 189–196
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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