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Photosensitization of bovine serum albumin by pterin: A mechanistic study

Paper ID Volume ID Publish Year Pages File Format Full-Text
30628 44494 2013 7 PDF Available
Title
Photosensitization of bovine serum albumin by pterin: A mechanistic study
Abstract

Pterins, heterocyclic compounds widespread in biological systems, are able to photoinduce oxidation of DNA and its components. In the present study, we have investigated the photosensitizing properties of pterin (Ptr), the parent compound of oxidized pterins, using bovine serum albumin (BSA) as target. Aqueous solutions of BSA were exposed to UV-A irradiation (350 nm) in the presence of Ptr, under various experimental conditions. The photosensitized processes were followed by UV/vis spectrophotometry, an enzymatic method for H2O2 determination and electrophoresis (SDS–PAGE). We present data that demonstrate unequivocally that BSA is damaged by Ptr. Although association between Ptr and the protein was evidenced by steady-state and time-resolved fluorescence measurements, the photosensitized damage takes place via a purely dynamic mechanism, which involves an electron transfer from BSA to the triplet excited state of Ptr, formed after UV-A excitation.

► Under UV-A radiation, bovine serum albumin (BSA) is damaged by pterin (Ptr). ► BSA and Ptr form a ground state complex that does not participate in the photosensitized reaction. ► The mechanism involves an electron transfer-initiated process. ► The photochemical process produces hydrogen peroxide.

Keywords
Pterins; Bovine serum albumin; Photosensitized processes; Reactive oxygen species; UV-A radiation
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Photosensitization of bovine serum albumin by pterin: A mechanistic study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 120, 5 March 2013, Pages 52–58
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us