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Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment

Paper ID Volume ID Publish Year Pages File Format Full-Text
30631 44494 2013 8 PDF Available
Title
Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment
Abstract

Photodynamic therapy (PDT) is a local tumour treatment accepted for a number of indications. PDT operates via the cellular stress response through the production of reactive oxygen species and subsequent cellular damage, resulting in cell death. Although PDT-induced signalling and cytotoxicity mechanisms have been investigated, the effect of PDT on microRNA (miRNA) expression is largely unknown. Therefore, we conducted a comprehensive microarray-based analysis of the miRNome of human epidermoid carcinoma cells (A431) following in vitro photodynamic treatment using polyvinylpyrrolidone hypericin (PVPH) as a photosensitiser and nearly homogeneous apoptosis-inducing conditions. Using microarray analysis we found eight miRNAs to be significantly differentially expressed 5 h post treatment compared with the baseline levels and three miRNAs with more than 2-fold differential expression that could be detected in 1 or 2 biological replicates. The verification of these results by quantitative RT–PCR including a detailed time-course revealed an up to 15-fold transient over-expression of miR-634, miR-1246, miR-1290 and miR-487b compared with the basal level. For these miRNAs, in silico mRNA target prediction yielded numerous target transcripts involved in the regulation of cell stress, apoptosis, cell adherence and proliferation. This study provides the first comprehensive miRNome analysis after PDT treatment and may help to develop novel miRNA-based therapeutic approaches to further increase the efficiency of PDT.

Graphical abstractMicroarray-based analysis of miRNA expression patterns confirmed by quantitative RT–PCR provides the initial comprehensive characterisation of the miRNome in cancer cells undergoing photodynamic therapy-induced apoptotic cell death.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A comprehensive miRNome analysis after photodynamic treatment (PDT) is provided. ► Five hour post PDT, 11 miRNAs show differential expression in the microarray analysis. ► Seven miRNAs could be confirmed by independent RT-qPCR, including miR-634 and -1290. ► Time-course RT-qPCR analyses revealed additional temporal dynamics of these miRNAs. ► miRNA target genes may be involved in various cell stress-related processes.

Keywords
Photodynamic therapy; microRNA; miRNome; Microarray; Apoptosis; Polyvinylpyrrolidone hypericin
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Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 120, 5 March 2013, Pages 74–81
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us