fulltext.study @t Gmail

The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)

Paper ID Volume ID Publish Year Pages File Format Full-Text
30643 44494 2013 6 PDF Available
Title
The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)
Abstract

The development of curative techniques which are selective for neoplasms is one of the main focal areas in cancer research. The mechanism of cell damage due to Zinc phthalocyanine (ZnPcSmix)-mediated photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) was assessed by inverted light microscopy for morphology, the Trypan blue exclusion assay and adenosine triphosphate (ATP) luminescence assay for cell viability, alamarBlue for proliferation, Lactate Dehydrogenase (LHD) membrane integrity for cytotoxicity and fluorescent microscopy for ZnPcSmix localization. Fluorescent microscopy revealed that ZnPcSmix was localized in both mitochondria and lysosomes, and PDT treated cells showed damaging structural changes and decreased cell viability and proliferation. The light-dependent ZnPcSmix displayed appreciable photosensitivity and the intensity of damage was directly related to its concentration.

► ZnPcSmix is an effective photodynamic agent in vitro. ► ZnPcSmix localizes in mitochondria, lysosomes and Golgi apparatus. ► ZnPcSmix in its inactive form, showed no apparent destructive effects in breast cancer cells.

Keywords
Breast cancer; Zinc phthalocyanine; Photodynamic therapy
First Page Preview
The primary subcellular localization of Zinc phthalocyanine and its cellular impact on viability, proliferation and structure of breast cancer cells (MCF-7)
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 120, 5 March 2013, Pages 171–176
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us