fulltext.study @t Gmail

Spectroscopic studies of the effects of anticancer drug mitoxantrone interaction with calf-thymus DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30644 44494 2013 6 PDF Available
Title
Spectroscopic studies of the effects of anticancer drug mitoxantrone interaction with calf-thymus DNA
Abstract

Mitoxantrone (MTX) (1,4-dihydroxy-5,8-bis[[2-[(2-hydroxyethyl)amino]ethyl]amino]-9,10-anthracenedione) is a synthetic antineoplastic drug, widely used as a potent chemotherapeutic agent in the treatment of various types of cancer. It is structurally similar to classical anthracyclines. Widespread interest in the anticancer agent mitoxantrone has arisen because of its apparent lower risk of cardio-toxic effects compared to the naturally occurring anthracyclines. In the present work, we investigated the interaction of mitoxantrone with DNA in the buffer solution at physiological pH using Fourier transform infrared (FTIR), UV–Visible absorption and circular dichroism spectroscopic techniques. FTIR analysis revealed the intercalation of mitoxantrone between the DNA base pairs along with its external binding with phosphate–sugar backbone. The binding constant calculated for mitoxantrone–DNA association was found to be 3.88 × 105 M−1 indicating high affinity of drug with DNA double helix. Circular dichroism spectroscopic results suggest that there are no major conformational changes in DNA upon interaction with drug except some perturbations in native B-DNA at local level. The present work shows the capability of spectroscopic analysis to characterize the nature of drug–biomolecule complex and the effects of such interaction on the structure of biomolecule.

Graphical abstractInteraction of mitoxantrone with DNA was investigated using Fourier transform infrared (FTIR), UV–Visible absorption and circular dichroism spectroscopic techniques. Results affirmed that mitoxantrone intercalates between the base pair of DNA.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Interaction of mitoxantrone with DNA was investigated using different spectroscopic techniques. ► Mitoxantrone intercalates between DNA base pairs. ► There is no major conformational change in DNA upon interaction with drug. ► The binding constant calculated for mitoxantrone–DNA association was found to be 3.88 × 105 M−1.

Keywords
Mitoxantrone; DNA–drug interaction; FTIR spectroscopy; CD spectroscopy
First Page Preview
Spectroscopic studies of the effects of anticancer drug mitoxantrone interaction with calf-thymus DNA
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 120, 5 March 2013, Pages 177–182
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us