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Contribution of reactive oxygen species to UV-B-induced damage in bacteria

Paper ID Volume ID Publish Year Pages File Format Full-Text
30652 44495 2012 7 PDF Available
Title
Contribution of reactive oxygen species to UV-B-induced damage in bacteria
Abstract

The present work aimed to identify the reactive oxygen species (ROS) produced during UV-B exposure and their biochemical targets, in a set of bacterial isolates displaying different UV susceptibilities. For that, specific exogenous ROS scavengers (catalase/CAT, superoxide dismutase/SOD, sodium azide and mannitol) were used. Biological effects were assessed from total bacterial number, colony counts and heterotrophic activity (glucose uptake and respiration). DNA strand breakage, ROS generation, oxidative damage to proteins and lipids were used as markers of oxidative stress. Sodium azide conferred a statistically significant protection in terms of lipid oxidation and cell survival, suggesting that singlet oxygen might play an important role in UV-B induced cell inactivation. Mannitol exerted a significant protection against DNA strand breakage and protein carbonylation, assigning hydroxyl radicals to DNA and protein damage. The addition of exogenous CAT and SOD significantly protected the capacity for glucose uptake and respiration, suggesting that superoxide and H2O2 are involved in the impairment of activity during UV-B exposure. The observation that amendment with ROS scavengers can sometimes also exert a pro-oxidant effect suggests that the intracellular oxidant status of the cell ultimately determines the efficiency of antioxidant defenses.

► ROS involved in UV-B damage in bacteria were identified using specific scavengers. ► Singlet oxygen participates in lipid peroxidation and inactivation by UV-B. ► Superoxide and hydrogen peroxide are involved in impairment of glucose incorporation and respiration. ► The hydroxyl radical is involved in DNA and protein oxidative damage. ► The oxidant status of the cell determines the efficiency of antioxidant defenses.

Keywords
Bacteria; UV radiation; Reactive oxygen species; Oxidative damage
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Contribution of reactive oxygen species to UV-B-induced damage in bacteria
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 117, 5 December 2012, Pages 40–46
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us