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Indicators of oxidative stress after ionizing and/or non-ionizing radiation: Superoxid dismutase and malondialdehyde

Paper ID Volume ID Publish Year Pages File Format Full-Text
30661 44495 2012 4 PDF Available
Title
Indicators of oxidative stress after ionizing and/or non-ionizing radiation: Superoxid dismutase and malondialdehyde
Abstract

Several authors have suggested that low level laser light may have a positive influence on side effects caused by ionizing radiation therapy. We therefore studied indicators of oxidative stress after exposure to gamma radiation with or without pre-exposure to low level laser light. Groups of mice were exposed to light from a laser diode at a wavelength of 830 nm, delivering an energy of 20 or 100 J to 1 cm2 in the abdominal part of the animal with a power density of 300 mW/cm2 in continuous regime. Following this treatment (or sham irradiation), mice were irradiated with graded doses of 60Co gamma rays. Levels of superoxide dismutase and malondialdehyde were measured in murine blood cells 30 min or 3 days after exposure. For both time points, there was a clear increase of superoxide dismutase and malondialdehyde with gamma dose, but laser light (alone or in combination with gamma irradiation) did not seem to have any influence on either parameter. Because the physical parameters in our experiments were similar to those of studies showing a positive effect of laser pre-exposure, we conclude that the lack of an observed effect in our case was due to differences in biological parameters, i.e. to differences between the tissues or cell types studied. It is also possible, of course, that laser effects would be seen mainly in the skin immediately exposed, and not to the same degree in blood cells circulating through that area, which were exposed to considerably smaller laser energies.

► We analyzed oxidative stress after 60Co and/or 830 nm therapeutic laser radiation. ► SOD, MDA and GSH/GSSG were determined in mice. ► We did find a clear increase of SOD and MDA with gamma dose. ► There was no significant radioprotective effect of the therapeutic laser.

Keywords
Oxidative stress; Malondialdehyde; Superoxide dismutase; Low level laser light
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Indicators of oxidative stress after ionizing and/or non-ionizing radiation: Superoxid dismutase and malondialdehyde
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 117, 5 December 2012, Pages 111–114
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us