fulltext.study @t Gmail

Spectroscopic studies on the binding of holmium-1,10-phenanthroline complex with DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30664 44495 2012 8 PDF Available
Title
Spectroscopic studies on the binding of holmium-1,10-phenanthroline complex with DNA
Abstract

Fluorescence and absorption spectroscopy, circular dichroism (CD) as well as viscosity experiment have been used to characterize the DNA binding of [Ho(Phen)2Cl3]·H2O, where phen stand for 1,10-phanathroline. This complex exhibits the marked decrease in the emission intensity and some hypochromism in UV–Vis spectrum in the presence of DNA. For characterization of the binding mode between the Ho(III) complex and DNA various procedures such as: absorption and emission titration and EB quenching experiments, viscosity measurements, CD study, iodide quenching assay, salt effect and thermodynamical investigation are used. The intrinsic binding constant of [Ho(Phen)2Cl3]·H2O with DNA is calculated by UV–Vis and florescence spectroscopy. The value of binding constants in 296, 299 and 303 are 1.99 ± 0.07 × 104, 1.07 ± 0.09 × 104 and 0.84 ± 0.06 × 104, respectively. The thermodynamic studies show that the reaction is entropically driven. The above-mentioned physical measurements indicate that the Ho(III) complex binds to fish salmon DNA, presumably via groove binding mode.

Graphical abstractIn this work, an attempt has been made to investigate the interaction of [Ho(Phen)2Cl3]·H2O with fish salmon DNA using UV–vis, fluorescence spectroscopy, circular dichroism and viscosimetery techniques.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Ho(III) complex shows good binding affinity to FS DNA with Kb = 1.07 × 104 M−1. ► Viscosity of DNA almost unchanged by increasing amount of Ho complex. ► CD spectrum of DNA has a little change with increasing amount of Ho complex. ► Thermodynamic parameters indicate that the binding reaction is entropically driven. ► Our studies suggest that the binding mode is groove binding.

Keywords
Holmium(III) complex; 1,10-Phenantroline; Fluorescence; DNA interaction; Thermodynamic parameters; Groove binding
First Page Preview
Spectroscopic studies on the binding of holmium-1,10-phenanthroline complex with DNA
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 117, 5 December 2012, Pages 132–139
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us