fulltext.study @t Gmail

Molecular aspects on the specific interaction of homoisoflavonoids to DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30674 44495 2012 7 PDF Available
Title
Molecular aspects on the specific interaction of homoisoflavonoids to DNA
Abstract

Homoisoflavonoids (3-benzylidenechroman-4-ones) are related to flavonoids and occur as natural products and exhibit biological activity. These compounds have been reported to possess antioxidant, antifungal, hypocholesterolemic, antimutagenic and antiviral activities. This study was designed to examine the interactions of four synthetic homoisoflavonoids (BMC, BPC, HBC and HBMC) with calf-thymus DNA in aqueous solution at physiological conditions, using constant DNA concentration (6.25 mM) and various homoisoflavonoids/polynucleotide (phosphate) ratios of 1/120, 1/80, 1/40, 1/20, 1/10 and 1/5. Fourier transform infrared (FTIR) and UV–Visible spectroscopic methods were used to determine the ligand binding modes, the binding constants and the stability of homoisoflavonoids–DNA complexes in aqueous solution. Spectroscopic evidence showed major binding of homoisoflavonoids to DNA with overall binding constants of KBMC–DNA = 9.37(±0.34) × 103 M−1, KBPC–DNA = 1.8(±0.09) × 104 M−1, KHBC–DNA = 1.3(±0.19) × 103 M−1 and KHBMC–DNA = 4.7(±0.41) × 103 M−1. The affinity of homoisoflavonoids–DNA binding is in the order of BPC > BMC > HBMC > HBC. No biopolymer secondary structural changes were observed upon homoisoflavonoids interaction and DNA remains in the B-family structure in these complexes.

► We model the binding of BMC, BPC, HBC and HBMC to DNA by using the spectroscopic methods. ► We investigate the effects of drug complexation on DNA stability and conformation. ► The affinity of homoisoflavonoids–DNA binding is in the order of BPC > BMC > HBMC > HBC. ► BMC, BPC, HBC and HBMC bind to DNA via a nonintercalation mode. ► No DNA conformational changes were observed upon homoisoflavonoids complexation.

Keywords
DNA; Homoisoflavonoids; 3-benzylidenechroman-4-ones; FTIR; UV–Visible spectroscopy
First Page Preview
Molecular aspects on the specific interaction of homoisoflavonoids to DNA
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 117, 5 December 2012, Pages 207–213
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us