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Fluorescence investigation of the interaction of 2-(4-fluorophenyl)-1-phenyl-1H-phenanthro [9,10-d] imidazole with bovine serum albumin

Paper ID Volume ID Publish Year Pages File Format Full-Text
30676 44495 2012 6 PDF Available
Title
Fluorescence investigation of the interaction of 2-(4-fluorophenyl)-1-phenyl-1H-phenanthro [9,10-d] imidazole with bovine serum albumin
Abstract

The interaction between 2-(4-fluorophenyl)-1-phenyl-1H-phenanthro [9,10-d] imidazole (FPPI) and bovine serum albumin (BSA) was investigated by fluorescence spectral studies. The observed experimental result shows that the imidazole derivative has strong ability to quench the fluorescence of BSA by forming complex which is stabilized by electrostatic interactions. The effective quenching constants (Ksv) were 2.78 × 104, 2.52 × 104, and 2.32 × 104 at 301, 310, and 318 K respectively. The Stern–Volmer quenching constant (Ksv), binding site number (n), apparent binding constant (KA) and corresponding thermodynamic parameters (ΔG, ΔH, and ΔS) were calculated. The distance between the donor (BSA) and acceptor (FPPI) was obtained according to fluorescence resonance energy transfer (FRET). Conformational changes of BSA were observed from synchronous fluorescence technique. The effect of metal ions such as Cu2+, Zn2+, Ca2+, Mg2+, Ni2+, Co2+, and Fe2+ on the binding constants between the FPPI and BSA were also studied.

Graphical abstract.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Interactions between FPPI and BSA were investigated by fluorescence quenching. ► Quenching mechanism arise from the formation of BSA–FPPI complex. ► Synchronous fluorescence spectra to exploit the structural change of BSA. ► The effect of other ions on the binding constants between FPPI and BSA.

Keywords
Phenanthroimidazole; BSA; Fluorescence; Quenching; Lifetime
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Fluorescence investigation of the interaction of 2-(4-fluorophenyl)-1-phenyl-1H-phenanthro [9,10-d] imidazole with bovine serum albumin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 117, 5 December 2012, Pages 222–227
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us