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Capture and intermediate purification of recombinant antibodies with combined precipitation methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
3071 149 2015 12 PDF Available
Title
Capture and intermediate purification of recombinant antibodies with combined precipitation methods
Abstract

•Combined precipitation methods with equal separation power as chromatographic methods.•Replacement of capture and intermediate steps by caprylic acid and PEG precipitation.•Combination of CEP, PEG, CA and CaCl2 precipitation to remove host cell impurities.•The final yield and purity of the antibodies nearly reached drug substance values.

A protocol for capturing recombinant antibodies was developed with a combination of different precipitation methods. This process comprised four different precipitations, including caprylic acid, polyethylene glycol (PEG), CaCl2, and cold ethanol. The final yield and purity of the antibodies nearly reached drug substance values, with a low host cell protein concentration (<300 ppm), no high molecular weight impurities, and a yield of 70%. In fact, only the combination of caprylic acid and PEG precipitations was needed to achieve purity similar to that produced with protein A affinity chromatography. Caprylic acid was used to remove the host cell proteins and high molecular weight impurities, and the PEG precipitation separated the IgG from the remaining impurities.

Keywords
Downstream processing; Purification; Monoclonal antibody; Ethanol; Polyethylene glycol; Precipitation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 93, 15 January 2015, Pages 200–211
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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