fulltext.study @t Gmail

Synthesis, characterization, biological studies (DNA binding, cleavage, antibacterial and topoisomerase I) and molecular docking of copper(II) benzimidazole complexes

Paper ID Volume ID Publish Year Pages File Format Full-Text
30737 44500 2012 12 PDF Available
Title
Synthesis, characterization, biological studies (DNA binding, cleavage, antibacterial and topoisomerase I) and molecular docking of copper(II) benzimidazole complexes
Abstract

To explore the therapeutic potential of copper-based benzimidazole complexes, tetranuclear Cu(II) complex 1 and dinuclear ternary amino acid complexes 2 and 3 {L-trp and L-val, respectively} were synthesized and thoroughly characterized. In vitro DNA binding studies of complexes 1–3 were carried out employing UV–vis titrations, fluorescence, circular dichroic and viscosity measurements which revealed that the complexes 1–3 bind to CT DNA preferably via groove binding. Complex 1 cleaved pBR322 DNA via hydrolytic pathway (validated by T4 DNA ligase assay), accessible to major groove while 2 followed oxidative mechanism, binding to minor groove of DNA double helix; binding events were further validated by molecular docking studies. Additionally, the complexes 1 and 2 exhibit high Topo-I inhibitory activity at different concentrations. The complexes 1–3 were evaluated for antibacterial activity against Escherichia coli and Staphylococcus aureus, and 2 was found to be most effective against Gram-positive bacteria.

Graphical abstractMolecular docking techniques were performed to elucidate the molecular recognition of multinuclear copper(II) complexes of benzimidazole scaffold towards the major/minor groove of DNA as Topo-I inhibitors.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Tetranuclear Cu(II) complex, 1 and dinuclear amino acid ternary benzimidazole complexes 2 and 3. ► In vitro DNA binding studies with CT DNA. ► Complex 1 cleaved pBR322 DNA via hydrolytic mechanism (validated by T4 DNA ligase). ► Complexes show high inhibition activity against Topo-I. ► Validation by molecular docking studies.

Keywords
Cu(II)-benzimidazole; In vitro DNA binding; Cleavage activity; Topo-I inhibitors; Groove binding; Molecular docking
First Page Preview
Synthesis, characterization, biological studies (DNA binding, cleavage, antibacterial and topoisomerase I) and molecular docking of copper(II) benzimidazole complexes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 114, 3 September 2012, Pages 15–26
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us