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Protein indicators for HaCaT cell damage induced by UVB irradiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
30746 44500 2012 8 PDF Available
Title
Protein indicators for HaCaT cell damage induced by UVB irradiation
Abstract

UVB (290–320 nm) is one major risk factor of skin diseases in human. In order to provide potential protein molecules in skin cell damage and skin diseases induced by UVB irradiation, the differentially expressed proteins in human keratinocytes cell HaCaT by UVB irradiation were screened by two-dimensional difference in-gel electrophoresis (2D DIGE) combined to high performance liquid chromatography-nano-electrospray ionization tandem mass spectrometry (HPLC-nESI-MS/MS). 31 protein spots were found differentially expressed with statistical significance (p < 0.05). Sixteen and 15 protein spots were observed up-regulated and down-regulated in UVB-irradiated HaCaT, respectively. Twenty-eight unique proteins were identified by searching the MS/MS data against NCBInr database through TurboSequest Bioworks software. Among the identified 28 UVB irradiation responding protein indicators, only laminin receptor 1 (RPSA), calmodulin (CALM3), cathepsin D (CTSD) and peroxiredoxin (PRDX1) proteins have been reported associated with skin burn and wound healing, keratinocytes proliferation and migration and epidermal barrier repairing. Most of these targets were for the first time revealed to be associated with skin cell damage induced by UVB irradiation. Function and bioinformatics analyses of the identified protein candidates were also performed using PANTHER analysis with the aid of DAVID platform. The current work provides potential protein indicators for skin cell damage from UVB irradiation.

► Human keratinocytes cells (HaCaTs) were stimulated by UVB irradiation. ► Proteomics results indicated 28 proteins were differentially expressed in HaCaT following UVB irradiation. ► Most of the identified protein candidates were for the first time revealed associated with skin cell damage.

Keywords
UVB; HaCaT; 2D DIGE; HPLC-nESI-MS/MS; Proteomics
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Protein indicators for HaCaT cell damage induced by UVB irradiation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 114, 3 September 2012, Pages 94–101
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us