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Synthesis of heterobimetallic complexes: In vitro DNA binding, cleavage and antimicrobial studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
30748 44500 2012 11 PDF Available
Title
Synthesis of heterobimetallic complexes: In vitro DNA binding, cleavage and antimicrobial studies
Abstract

The monometallic (1–3) and heterobimetallic (4–6) complexes, containing 1,10-phenanthroline and indole-3-acetic acid were synthesized and characterized by spectroscopic (IR, UV–vis, NMR, ESI-MS) and analytical methods. The in vitro DNA binding studies of 5 and 6 with CT DNA were carried out by employing various biophysical methods which reveal strong electrostatic binding via phosphate backbone of DNA helix. The binding constant (K) value as determined from fluorescence experiments of complexes 5 and 6 were calculated to be 4.09 × 104 and 2.51 × 104 M−1, respectively revealing that complex 5 has greater binding propensity for DNA. To gain further insight into the molecular recognition at the target site, interaction studies of 5 with 5′-GMP were carried out by employing 1H and 31P NMR spectroscopy. Complex 5 exhibited preferential selectively towards the minor groove of pBR322 DNA and efficient cleavage activity via hydrolytic pathway. Furthermore complexes 4–6 exhibited significant antimicrobial activity.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► New CuII–Sn2IV and CoII–Sn2IV complexes were synthesized and characterized. ► DNA binding studies revealed the electrostatic interaction into the minor groove. ► Complex 5 shows efficient hydrolytic cleavage of pBR322 DNA. ► Complex 5 exhibited significant antimicrobial activity.

Keywords
CT DNA binding; 5′-GMP; DNA cleavage; Antimicrobial activity
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Synthesis of heterobimetallic complexes: In vitro DNA binding, cleavage and antimicrobial studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 114, 3 September 2012, Pages 108–118
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us