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Investigating the interaction between the violaxanthin cycle enzyme zeaxanthin epoxidase and the thylakoid membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
30749 44500 2012 7 PDF Available
Title
Investigating the interaction between the violaxanthin cycle enzyme zeaxanthin epoxidase and the thylakoid membrane
Abstract

In the present study the interaction between the violaxanthin cycle enzyme zeaxanthin epoxidase (ZEP) and the thylakoid membrane was investigated. Isolated, active thylakoid membranes of spinach (Spinacia oleracea L.) were subjected to different salt and detergent treatments that are generally used to isolate peripheral and integral membrane proteins. These salt and detergent treatments included the use of the salts NaBr, Na2CO3 and Tris and the detergents octylglucoside (OG) and dodecylmaltoside (DM). After the treatments the activity of the ZEP was determined in washed thylakoid membranes. To obtain additional information about the mode of ZEP binding to the membrane a hydrophobicity plot based on the amino acid sequence of the protein was constructed. The plot was then compared to a diagram obtained for the photosystem II antenna Lhcb1 protein whose integration into the thylakoid membrane is known. The results of the salt and detergent treatments of the thylakoid membrane suggest that the ZEP is a peripheral, rather weakly bound membrane protein. Results from the hydrophobicity plots indicate the existence of specialized protein domains which may realize the partial integration and binding of the ZEP to the thylakoid membrane.

► Zeaxanthin epoxidase (ZEP) is a peripheral membrane protein. ► ZEP is located at the stromal side of the thylakoid membrane. ► ZEP is constitutively but rather weakly bound. ► Binding is probably realized by a helix-like protein domain.

Keywords
Membrane protein; Thylakoid membrane; Violaxanthin cycle; Zeaxanthin epoxidase
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Investigating the interaction between the violaxanthin cycle enzyme zeaxanthin epoxidase and the thylakoid membrane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 114, 3 September 2012, Pages 119–125
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us