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2D measurement of ion currents associated to the signal transduction of the phototactic alga Chlamydomonas reinhardtii

Paper ID Volume ID Publish Year Pages File Format Full-Text
30753 44500 2012 6 PDF Available
Title
2D measurement of ion currents associated to the signal transduction of the phototactic alga Chlamydomonas reinhardtii
Abstract

Our objective was to develop a simple procedure for the detection of light-induced ion currents of photomotile cells in two dimensions. The novel technique was based on the light gradient method (LGM), and the model object was Chlamydomonas reinhardtii, a phototactic unicellular alga, ideal for such experiments. The conventional LGM cuvette was modified such that the electrode pair could be rotated around the sample and pick up the electric signals from arbitrary directions. The experiments were performed with and without the application of an auxiliary light beam preorienting the motile cells. The analysis of the detected traces revealed two main vectorial components of the signal by the help of singular value decomposition (SVD), in concert with previous experimental findings and theoretical considerations suggesting different origins of the “fast” and “slow” components of the photoelectric response of Chlamydomonas and Haematococcus cells. Using plausible assumptions, our method allowed a quantitative analysis of the signal, assigning size and direction to the two vectorial components. The method allows a rapid and accurate way to measure electric signals of photomotive cells in 2D, and particularly to test the physiological activity and in vivo-kinetics of site-directed mutants of ChR1 or ChR2, providing novel photo-electrophysiological methods with important quantitative information.

► Measurement of light-induced ion currents of photomotile cells in two dimensions. ► A quantitative analysis of the signals assigns size and direction to the two vectorial components. ► Utilization chances are expected in selecting new pigments for optogenetic applications.

Keywords
Electric measurement; Phototaxis; Chlamydomonas; Channelrhodopsins; Optogenetics
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2D measurement of ion currents associated to the signal transduction of the phototactic alga Chlamydomonas reinhardtii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 114, 3 September 2012, Pages 147–152
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us