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Real-time detection of dental calculus by blue-LED-induced fluorescence spectroscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
30758 44501 2007 7 PDF Available
Title
Real-time detection of dental calculus by blue-LED-induced fluorescence spectroscopy
Abstract

Successful periodontal therapy requires sensitive techniques to discriminate dental calculus from healthy teeth. The aim of the present study was to develop a fluorescence-based procedure to enable real-time detection and quantification of dental calculus. Thirty human teeth – 15 teeth with sub- and supragingival calculus and 15 healthy teeth – covered with a layer of physiological saline solution or blood were illuminated by a focused blue LED light source of 405 nm. Autofluorescence spectra recorded along a randomly selected line stretching over the crown-neck-root area of each tooth were utilized to evaluate a so called calculus parameter R, which was selected to define a relationship between the integrated intensities specific for healthy teeth and for calculus in the 477–497 nm (SA) and 628–685 nm (SB) wavelength regions, respectively. Statistical analysis was performed and a cut-off threshold of R = 0.2 was found to distinguish dental calculus from healthy teeth with 100% sensitivity and specificity under various experimental conditions. The results of the spectral evaluation were confirmed by clinical and histological findings. Automated real-time detection and diagnostics for clinical use were implemented by a corresponding software program written in Visual Basic language. The method enables cost-effective and reliable calculus detection, and can be further developed for imaging applications.

Keywords
Fluorescence spectroscopy; Dental calculus; Light-emitting diode; Diagnosis
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Real-time detection of dental calculus by blue-LED-induced fluorescence spectroscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 87, Issue 2, 25 May 2007, Pages 88–94
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us