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Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
3077 149 2015 10 PDF Available
Title
Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures
Abstract

Biopolymers, such as polyhydroxyalkanoates (PHA), are an environmentally friendly alternative to plastics derived from fossil fuels. However, producing PHA in a cost-effective way requires the development of highly efficient separation and purification treatments. In this study, one acid treatment (sulphuric acid combined with a subsequent bleaching step) and three alkaline treatments (sodium hypochlorite, sodium hydroxide and a combination of the latter with an halogenated solvent) were evaluated for recovering poly(3-hydroxybutyrate) (PHB), a type of PHA, from Cupriavidus necator H16 cells with high biopolymer content (65%). Purity, percent of recovery and the properties of the PHB obtained after each treatment, together with the costs and environmental impacts associated with each treatment, were determined and compared. The lowest recovery costs were obtained with the sodium hydroxide and sulphuric acid treatments (1.02 and 1.11 € kg−1, respectively). Estimated CO2 emissions of these two treatments were 18% of those based on the use of sodium hypochlorite. However, the highest purity (98%) and lowest polymer degradation were achieved with the acid treatment. Consequently, the acid treatment was selected as the most effective choice for PHA recovery.

Keywords
Polyhydroxyalkanoates; Cupriavidus necator; Purification; Bioseparation; Cell disruption; Downstream processing
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Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 93, 15 January 2015, Pages 250–259
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us