fulltext.study @t Gmail

Analysis of the role of Mg2+ on conformational change and target recognition by Ciliate Euplotes octocarinatus centrin

Paper ID Volume ID Publish Year Pages File Format Full-Text
30774 44502 2011 9 PDF Available
Title
Analysis of the role of Mg2+ on conformational change and target recognition by Ciliate Euplotes octocarinatus centrin
Abstract

The binding of Mg2+ with the Euplotes octocarinatus centrin (EoCen) and the effect of Mg2+ on the binding of EoCen with the peptide melittin were examined by spectroscopic methods. In this study, it was found that Mg2+ may bind with Ca2+-binding sites, at least partly, on EoCen, which displays ∼10-fold weaker affinity than Ca2+. In the presence of Mg2+, Ca2+-saturated EoCen undergoes significant conformational changes resulting in decreased exposure of hydrophobic surfaces on the protein. Additionally, excess Mg2+ did not change the stoichiometry, but rather reduced the affinity of EoCen to melittin. The Mg2+-dependent decrease in the affinities of EoCen to melittin is an intrinsic property of Mg2+, rather than a nonspecific ionic effect. The inhibitory effect of Mg2+ on the formation of complexes between EoCen and melittin may contribute to the specificity of EoCen in target activation in response to cellular Ca2+ concentration fluctuations.

► Mg2+ may bind with EoCen and located, at least partially, within the calcium-binding sites. ► Mg2+ plays an opposite role to the Ca2+-induced conformations changes for EoCen. ► Mg2+ decreased the affinities of mimic peptide melittin with EoCen.

Keywords
Centrin; Magnesium; Melittin; Conformational change
First Page Preview
Analysis of the role of Mg2+ on conformational change and target recognition by Ciliate Euplotes octocarinatus centrin
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 105, Issue 1, 5 October 2011, Pages 60–68
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us