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The effect of Se salts on DNA structure

Paper ID Volume ID Publish Year Pages File Format Full-Text
30789 44503 2012 6 PDF Available
Title
The effect of Se salts on DNA structure
Abstract

There is considerable interest in the role of selenium in cancer prevention. Various organic and inorganic Se compounds are considered to be antioxidants. In the present study, the binding modes, the binding constants and the stability of Se–DNA complexes have been determined by Fourier transform infrared (FTIR) and UV–Visible spectroscopic methods. Spectroscopic evidence showed that Na2SeO4 and Na2SeO3 bind to the minor and major grooves of DNA and the backbone phosphate (PO2) with overall binding constants of K(Na2SeO4–DNA) = 5.20 × 104 M−1 and K(Na2SeO3–DNA) = 1.87 × 103 M−1. DNA aggregations occurred at high selenium concentrations. No biopolymer conformational changes were observed upon Na2SeO3 and Na2SeO4 interactions, while DNA remained in the B-family structure.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The binding affinity of Na2SeO4 and Na2SeO3 to DNA was determined. ► The binding constant of Na2SeO4–DNA complexes is higher than Na2SeO3–DNA complexes. ► Na2SeO4 and Na2SeO3 mainly interact with G, A, T and backbone PO2 group. ► DNA aggregation occurred at higher selenium salts concentrations.

Keywords
DNA; Sodium selenate; Sodium selenite; FTIR spectroscopy; UV–Visible
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The effect of Se salts on DNA structure
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 113, 1 August 2012, Pages 36–41
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us