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Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents

Paper ID Volume ID Publish Year Pages File Format Full-Text
3079 149 2015 7 PDF Available
Title
Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents
Abstract

•Immobilization of lipases by adsorption on ionic and hydrophobic supports.•Evaluation of the effect of solvents and surfactants on enzymatic activity of immobilized lipases.•Study of effect of solvents and surfactants on the enzyme structure (opened and closed conformation).•Evaluation of selectivity of immobilized lipases on the hydrolysis of fish oil.

Most of lipases are in equilibrium between a majority inactive closed form and a minority active open form in aqueous media. Perhaps, a certain stabilization of these open forms of lipases could be achieved in the presence of cosolvents or surfactants in the reaction medium. Three commercial lipases were studied (from Thermomyces lanuginosa (TLL), Candida Antarctica fraction B (CALB) and Lecitase (LEC)). Different derivatives were tested: TLL and LEC were adsorbed on an anionic exchanger and their activity strongly depends on the equilibrium between their open and closed form and CALB was adsorbed on a hydrophobic support when the open form was already stabilized by the support. Derivatives ionically adsorbed were hyperactivated by surfactans as well as by cosolvents: the activity of LEC increased 12 times in the presence of 15–20% of ethanol. CALB adsorbed on hydrophobic supports was hardly hyperactivated and even it was inhibited. The modification of the rate of covalent modification of the catalytic Ser seems to confirm that the observed hyperactivations were due to a stabilization of the open form of the adsorbed lipases (TLL and LEC). The hydrolysis of sardine oil was also studied in the presence or absence of surfactants and cosolvents. An interesting improvement in the ability of derivatives to discriminate the release of eicosipentaenic acid (EPA) and docosahexaenicacid (DHA) was found.

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Keywords
Lipases; Immobilization; Stability; Selectivity; Surfactants; Hydrolysis of fish oil
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Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 93, 15 January 2015, Pages 274–280
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us