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Resveratrol-sensitized UVA induced apoptosis in human keratinocytes through mitochondrial oxidative stress and pore opening

Paper ID Volume ID Publish Year Pages File Format Full-Text
30790 44503 2012 9 PDF Available
Title
Resveratrol-sensitized UVA induced apoptosis in human keratinocytes through mitochondrial oxidative stress and pore opening
Abstract

Resveratrol (3,5,4’-trihydroxy-trans-stilbene), a polyphenol compound, is derived from natural products such as the skin of red grapes, blueberries and cranberries. Resveratrol not only exhibits antioxidant, cardioprotection, and anti-aging properties, but can also inhibit cancer cell growth and induce apoptosis. It has been shown that resveratrol inhibits the activation of Nf-κB and subsequently down regulates the expression of Nf-κB regulated genes such as interleukin-2 and Bcl-2, leading to cell cycle arrest and increased apoptosis in multiple myeloma cells. In the skin, resveratrol has been reported to sensitize keratinocytes to UVA induced apoptosis. However, the effect of resveratrol on opening of the mitochondrial permeability transition pore has not been previously examined. Our data show that UVA (14 J/cm2) along with resveratrol causes massive oxidative stress in mitochondria. As a consequence of oxidative stress, the mitochondrial membrane potential decreases which results in opening of the mitochondrial pores ultimately leading to apoptosis in human keratinocytes. These results may have clinical implications for development of future chemotherapeutic treatment for tumors of the skin.

► Resveratrol in combination with UVA sensitized apoptosis in human keratinocytes. ► MMP was more dissipated in cells exposed to UVA and cultured with resveratrol. ► Combination treatment of UVA and resveratrol caused more active MPTP in cells. ► Higher ROS levels were detected in cells after the treatment with resveratrol and UVA. ► Disorganized mitochondrial structure was found after UVA and resveratrol treatment.

Keywords
Resveratrol; Keratinocyte; UVA induced apoptosis; Mitochondrial membrane potential; Mitochondrial permeability transition pore; Reactive oxygen species
First Page Preview
Resveratrol-sensitized UVA induced apoptosis in human keratinocytes through mitochondrial oxidative stress and pore opening
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 113, 1 August 2012, Pages 42–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering