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Fluorescence study on aggregated lysozyme and lipid bilayer interactions

Paper ID Volume ID Publish Year Pages File Format Full-Text
30791 44503 2012 5 PDF Available
Title
Fluorescence study on aggregated lysozyme and lipid bilayer interactions
Abstract

Fluorescent probes 1,6-diphenyl-1,3,5-hexatriene (DPH), pyrene, 4-dimethylaminochalcone (DMC) and 4-p-(dimethylaminostyryl)-1-dodecylpyridinium (DSP-12) have been utilized to monitor the impact of lysozyme (Lz) oligomers on physicochemical properties of phosphatidylcholine/cardiolipin (PC/CL) membranes. Analysis of spectral responses of the employed probes revealed the reduction of membrane free volume and dehydration of lipid bilayer surface upon incorporation of Lz self-assemblies. Hydrophobic interactions were found to control the binding of Lz oligomers to the lipid bilayer. Comparison of the effects of Lz monomers, oligomers and fibrils showed that soluble oligomeric intermediates exert the most destructive influence on membrane properties.

► Protein fibrillization alters its bilayer-modifying properties. ► Soluble lysozyme oligomers produce dehydration and condensation of lipid bilayer. ► Membranotropic activity of oligomeric lysozyme is hydrophobically controlled.

Keywords
Lysozyme oligomers; Lipid bilayer; Amyloid; Fluorescent probes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 113, 1 August 2012, Pages 51–55
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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