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Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30802 44504 2006 8 PDF Available
Title
Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA
Abstract

The influence of denaturation on DNA photochemistry was studied by quantifying the yield of formation of all possible bipyrimidine photolesions within isolated genomic DNA samples exposed to UVC radiation. Effects of DNA melting was studied either by carrying out irradiation over a wide range of temperature (0–90 °C) or by decreasing the ionic strength of the solution at 30 °C. A first observation was a much larger decrease in the photoreactivity upon increasing the temperature in single-stranded than in double-stranded DNA. Secondly, formation of trans,syn cyclobutane dimers and, to a lesser extent, modification in the ratio between the yields of cyclobutane dimers and (6-4) photoproducts, were found to be other main features associated with denaturation. These results emphasize the modulating role of structure in the yield and nature of UV-induced DNA damage.

Keywords
DNA damage; UV radiation; Pyrimidine dimers; DNA photochemistry; Ionic strength; DNA structure
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Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 82, Issue 1, 2 January 2006, Pages 45–52
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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