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Molecular spectroscopic studies on the interaction of morin with bovine serum albumin

Paper ID Volume ID Publish Year Pages File Format Full-Text
30809 44505 2012 7 PDF Available
Title
Molecular spectroscopic studies on the interaction of morin with bovine serum albumin
Abstract

The interaction between morin and bovine serum albumin (BSA) was studied using molecular spectroscopic approach at different temperatures under imitated physiological conditions. Quenching of intrinsic tryptophanyl fluorescence of BSA with increasing morin concentration is the actuating tool in the analysis. The obtained quenching mechanisms, binding constants, binding sites and corresponding thermodynamic parameters at different temperatures indicate that the hydrophobic interaction play a major role in the morin-BSA association. Binding affinity between morin and BSA was determined using Scatchard equation and the modified Stern–Volmer equation, and the corresponding Structure–affinity relationships of flavonoids were discussed. Site marker competitive displacement experiments demonstrated that morin binds with high affinity to site II (subdomain IIIA) of BSA. Furthermore, the circular dichroism spectral results indicated that the conformation of BSA changed in the presence of morin. In addition, the effect of some common metal ions on the binding constant between morin and BSA was examined.

► The binding mechanisms of morin to BSA at molecular lever was provided. ► The bind parameters, binding modes, binding site, and effect of metal ions have been explored. ► The structure-affinity relationships of flavonoids was undertaken to unravel. ► This study will help to design therapeutic drugs with more efficient than current ones.

Keywords
Morin; Bovine serum albumin; Conformational investigation; Binding parameters; Structure–affinity relationship
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 112, 2 July 2012, Pages 16–22
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us