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Micro-Raman spectroscopy study of the effect of Mid-Ultraviolet radiation on erythrocyte membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
30812 44505 2012 6 PDF Available
Title
Micro-Raman spectroscopy study of the effect of Mid-Ultraviolet radiation on erythrocyte membrane
Abstract

Mid-Ultraviolet (UVB) has a significant influence on human health. In this study, human erythrocytes were exposed to UVB to investigate the effects of UVB radiation on erythrocytes membrane. And Micro-Raman spectroscopy was employed to detect the damage. Principal component analysis (PCA) was used to classify the control erythrocytes and the irradiated erythrocytes. Results showed that the erythrocytes membrane was damaged by Mid-Ultraviolet (UVB) radiation. The intensity of the Raman peaks at 1126 cm−1 and 1082 cm−1 were used to calculate the Longitudinal Order-Parameters in Chains (Strans) which can present the liquidity and ionic permeability of erythrocyte membrane. After UVB radiation for 30 min, both the liquidity and ionic permeability decreased. At the same time, the intensity of the peaks at 1302 cm−1 (α-helix), 1254 cm−1 (random coil), 1452 cm−1 and 1430 cm−1 (CH2/CH3 stretch) have also changed which indicated the membrane protein also been damaged by UVB. In the whole process of radiation, the more UVB radiation dose the more damage on the erythrocyte membrane.

► We did Mid-Ultraviolet (UVB) radiation on human erythrocytes. ► Different UVB radiation does were adopted. ► After radiation, erythrocyte membrane was extracted. ► Micro-Raman spectroscopy was used to detect the damage on erythrocyte membrane. ► This result offers a strong base about the blood disease and diagnosis for clinical.

Keywords
Raman spectra; Erythrocyte membrane; UVB radiation; UVB damage
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Micro-Raman spectroscopy study of the effect of Mid-Ultraviolet radiation on erythrocyte membrane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 112, 2 July 2012, Pages 37–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us