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Performance of a fixed-bed solid-state fermentation bioreactor with forced aeration for the production of hydrolases by Aspergillus awamori

Paper ID Volume ID Publish Year Pages File Format Full-Text
3082 149 2015 6 PDF Available
Title
Performance of a fixed-bed solid-state fermentation bioreactor with forced aeration for the production of hydrolases by Aspergillus awamori
Abstract

•Amylases, cellulases, xylanases and proteases were produced by solid-state fermentation (SSF).•A fixed-bed bioreactor with forced aeration was efficiently used for enzyme production.•Despite temperature gradients, significant enzyme activities were obtained.

In order to overcome scale-up limitations of conventional tray-type solid-state fermentation (SSF) bioreactors, a cylindrical fixed-bed bioreactor with forced aeration was investigated for the production of a pool of industrially relevant enzymes by Aspergillus awamori IOC-3914 using babassu cake as raw material. Significant internal radial and axial temperature gradients were observed (up to 2.4 °C cm−1), but despite of this, good titers of the production of the six enzyme groups evaluated was observed in all three bed layers sampled. Maximum activities of exoamylases, endoamylases, proteases, xylanases and cellulases (CMCase) were, respectively, 73.4, 55.7, 31.8, 23.8 and 6.2 U g−1. Moreover, a considerable production of isoamylases (27.0 U g−1) was observed, although the production of starch-debranching enzymes by SSF is rarely reported. Productivities were highest for endoamylases (0.95 U g−1 h−1) and exoamylases (0.67 U g−1 h−1). The present results show that despite considerable axial and radial temperature gradients, significant levels of different enzymes can be obtained, giving evidence that fixed-bed bioreactors with forced aeration present a promising alternative in terms of instrumented bioreactors for SSF processes.

Keywords
Production of amylases; proteases, xylanases and cellulases; Solid-state fermentation; Fixed-bed bioreactors; Filamentous fungi; Temperature gradients and heat transfer
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Performance of a fixed-bed solid-state fermentation bioreactor with forced aeration for the production of hydrolases by Aspergillus awamori
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 93, 15 January 2015, Pages 303–308
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us